• Learn about the pros and cons of different approaches to genome engineering
• Gain insight into delivery methods and their optimisation
• Understand the importance of optimal guideRNA (and ssODN) design
• Understand techniques for ensuring verified clonal cultures are achieved
The recent development of various genome-engineering technologies has the potential to revolutionise our understanding of disease-causing molecular and genetic mechanisms. When used in combination with human induced pluripotent stem cells (hiPSCs), these technologies allow unprecedented insight on a patient/tissue specific basis. However, to apply genome-engineering technologies successfully in your lab several aspects need to be considered and optimised. In this webinar we discuss different approaches to genome-engineering and identify important parameters for effective hiPSC genome-engineering from cell culture and reagent delivery to novel approaches for single-cell cloning to establish true clonal cultures.
The webinar will be led by Narasimha Telugu, PhD, who is a scientist at the Pluripotent Stem Cell Technology Platform at the Max Delbrueck Center for Molecular Medicine in the Helmholtz Association, Berlin. He will share his best practices for fast, efficient and automated genome engineering in iPSCs. Katia Mattis (D.Phil) will provide an overview of how novel technology, developed by iotaSciences, can help support and automate critical aspects in every genome engineering project.