Smilla K. Maierhof, Christian Schinke, Janine Cernoch, Lois Hew, Laura Pauline Bruske, Valeria Fernandez Vallone, Kristin Fischer, Harald Stachelscheid, Petra Huehnchen, Matthias Endres, Sebastian Diecke, Narasimha Swamy Telugu, Wolfgang Boehmerle
NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease.
Stem Cell Research 73 (2023) 103253
