CRISPR-Cas9 gene editing – survey report CRISPR-Cas9 gene editing has been revolutionizing the scientific landscape ever since its discovery and positioning as a gene editing tool. It allows scientists to change (“edit”) genomic DNA sequences within cells precisely. The ease-of-use compared to other gene editing technologies, such as Zinc-fingers and...

Induced pluripotent stem cells – cell culture survey insights Induced pluripotent stem cells (iPSCs) have emerged as a revolutionary tool in biomedical research due to their ability to differentiate into various cell types, mirroring the development of tissues and organs in the human body. This versatility holds immense promise for...

Stem cell terminology What is a ‘blastocyst’ and what does ‘omnipotent’ mean? New to the world of stem cells? Learn and review common abbreviations and definitions in the field of stem cells. Need a reminder about key terminology for genome-editing? Explore common genome-editing terminology with our genome-editing glossary. A Adult...

Genome-editing terminology What is ‘Base Editing’ and what does ‘dCas9’ mean? New to the world of genome-editing? Learn and review common abbreviations and definitions in the field of genome-engineering here. Need a reminder about key terminology for stem cells? Explore common stem cell terminology with our stem cell glossary. A...

Introduction: Induced pluripotent stem cells (iPSCs) have revolutionized the field of regenerative medicine and disease modeling due to their ability to differentiate into various cell types. However, maintaining iPSCs in culture requires careful attention to detail and adherence to best practices. This guide aims to provide tips for a successful...

Mastering Single-Cell Cloning Achieving efficient and consistent single-cell cloning requires awareness of various factors that can make or break its outcome. Here, we share important tips and considerations for succeeding in single-cell cloning, covering culture medium selection, choice of culture dishes, cell health, contamination prevention and more. Culture medium The...

Introduction Biology is inherently complex. No single cell is exactly like any other with diversity and heterogeneity being a hallmark and driving force of life itself. However, often it is vital to separate cells with desirable traits from the rest of the pack. Single-cell cloning is a technique used in...

Understanding Single-Cell Cloning The ability to work with cultures derived from single cells enables researchers to mitigate potential confounding factors in the form of genetic or behavioural heterogeneity, establishing a foundation for reproducible experimental outcomes. Moreover, cell-based production processes often utilizes cultures that originated from a single cell. This supports...

The CRISPR-Cas system is a key gene editing tool that has revolutionised how we manipulate DNA, providing the simplest, most efficient, and adaptable approach yet to making gene edits. These systems – originating in bacteria as a form of adaptive immunity – can be categorised into six types based on...