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GRID Technology

What are GRIDs?

  • 256 cell-culture chambers
  • 1.8 mm x 1.8 mm area per chamber
  • < 1 µl working volume per chamber
Single-cell cloning in optically clear chambers and small volumes
GRIDs comprise the core of an automated and streamlined single-cell isolation and cloning workflow developed by iotaSciences. The chambers' small size and optical clarity allows highy reliable in-chamber verification of clonality with the isoHub directly after plating, while the isoCell or isoPick automate all tedious liquid handling steps.
Image of a GRID chamber containing a single cell

How are GRIDs made?

Chamber Formation

isoCell's microjet technology carves individual chambers out of immiscible liquids
A thin film of cell media (or stem-cell coatings) is reshaped using a fluid microjet of FC40STAR to create an array of 256 chambers on a 6 cm dish. The approach exploits interfacial tension and chambers are separated, throughout the entire workflow, by FC40STAR - an immiscible fluorocarbon.

Dispensing Cells

Isolate up to 94 single cells in a single 6 cm dish
Your cells are automatically dispensed in nanoliter volumes into the individual GRID chambers. Single cells are clearly visible in the GRID chambers through a standard microscope (4x objective) due to the optical clarity and small chamber size. This enables easy verification of monoclonality immediately after dispensing.

GRID benefits

Features GRID-chambers Well-plate Flat-bottom dish
Features GRID-chambers
Physically separated cell environments
Scalable
One-step surface coating
Single cells visible at edges
Single cells visible directly after plating
Low reagent consumption

Publications and Other Resources

Explore our publications which explain the technology in more detail.
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