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The modular Cloning Platform delivers a streamlined single-cell cloning workflow featuring ultra-low volume culture chambers.
The isoCell instrument automates all liquid-handling. It dispenses cells, exchanges culture medium and harvests clonal cultures for downstream applications — without the need for tedious manual pipetting. With the isoCell, cells are handled using low pressure and low shear forces. Together with optional heating it delivers exceptional cell viability. And, all of this functionality sits within the most compact system on the market — the isoCell fits neatly into any cell culture hood without obstructing ongoing cell culture work. Now you can unlock consistent results in every experiment.
After single cells are plated into GRID chambers they are imaged using the isoHub. Entire GRID chambers can be easily reviewed in the absence of optical edge effects typically associated with conventional culture plasticware. That way, you get absolute confidence in identifying chambers that contain a single cell. The isoHub also automates navigation through GRIDs and allows tracking of your selected clones, either using brightfield or fluorescent mode. What’s more, automated wireless data-transfer between the isoCell and isoHub guarantees a seamless and efficient workflow.
The Image Capture System lets you document monoclonality and clonal outgrowth in a single click using whole-chamber imaging. Simply select GRID chambers that contain a single cell, and the imaging system records and documents your selection with a whole-chamber image. Users can easily take follow-up images of relevant chambers throughout the process to document clonal outgrowth. Moreover, image files are auto-named and sorted according to the chamber's details, providing a start-to-finish history of each single cell, for a fully transparent process record.
The Cloning Platform features a CoolLED pE-4000 illumination system for fluorescence microscopy. Easily detect commonly used fluorescent molecules (GFP, RFP, DAPI, and more) thanks to the system’s 4 channels, 16 LED wavelengths, and a user-friendly control pad.
The system works equally well with adherent- and suspension cells. Several diverse cell types have been cloned successfully in GRIDs, including HEK, K562, Raji, CHO-K1, HT-1080, CHO-S, HCT116, various iPSC lines, embryonic stem cells and many more.
The Cloning Platform requires two separate consumables:
The isoCell is more than a dispenser. It also exchanges culture medium and harvests monoclonal cell lines from selected low-volume culture chambers in just a few clicks.
With just 210 x 230 x 300 mm, and a weight of 4.3 kg, the isoCell is extremely compact and easily fits inside any laminar flow hood, without obstructing other ongoing cell culture work.
Absolutely! The Cloning Platform is routinely used by our customers with cells requiring coatings. For specific questions on this, please get in touch with us.
All liquid handling requirements are performed by the isoCell, including dispensing cells, feeding and harvesting clonal colonies. It only requires the placement of a respective dish onto the isoCell’s bed and selecting the appropriate program on the touch screen.
Definitely – we offer different ways for trialling the system at your premises. Please get in touch with us to discuss the options.
You can find more information about the Cloning Platform in our resources as well as by directly contacting us – we are happy to answer all your questions!
Want to find out more about our single-cell platforms? Contact one of our knowledgeable experts today, visit our resources page, or request a demo to see the platforms in action.